Microbiota Analysis at the Single Cell Level in Minutes.

Discover a future where fast and easy understanding microorganisms at the point-of-need transforms our world.

We are Pushing the Boundaries of Molecular Biology to Pave the Way for Predictive and Preventive Analyses at the Point-of-Need

sample

One sample

test

One test

analysis

All microorganisms in
a single analysis

A comprehensive microbial mapping at the single cell scale

Species, activity and behavior
of each microorganism

Fully detailed results in a few minutes

Designed to be deployed directly
at sample collection sites

Chronos

How does Chronos work?

The platform combines real-time RT-qPCR and digital PCR in a single-test format. It delivers results in minutes at each stage of wine and beer production, in analyzing the presence of pathogen microorganisms in  human microbiota samples.

Its speed allows ensuring regular monitoring to prevent risks of contamination or deviations and make the right decisions quickly.

Microwave Lyser

Lysing in just ten seconds.

That's all you need to extract nucleic acids from your samples.

How does the Microwave Lyser work?

Regardless of the matrix type, thanks to its microwave technology, our Microwave Lyser guarantees fast and efficient extraction of DNA and RNA. The Microwave Lyser device is designed to drastically reduce hands-on time and easily integrates into your workflow.

A Truly Versatile Solution
to Predict Both Emerging Biothreats and Bioprotection

Biothreats

Level of activity of all dangerous microorganisms at the single cell level.

Bioprotection

Presence of protective microorganisms communities
at the single cell level.

Fight antimicrobial resistances

Improve quality production

Reduce chemical inputs

Elminiate biohazards

A Major Breakthrough in Microbiological Analyses with Real-World Applications

Medical

Prevent risks of infectious diseases and antimicrobial resistances.

Defense

Fight fast and efficiently against risks of biothreats.

Water

Prevent risks of sanitary contamination in bathing water.

Food & Beverage

Prevent microbiological contamination risks and evaluate the quality of bioprotection.

Agriculture

Prevent crops diseases, reduce chemical inputs and production loss.

We can adapt our solution to any applications where fast and accurate analysis of microorganisms is essential →

Game-Changing Technological Breakthrough Over a Decade of R&D and 29 Registered Patents

MPD (Molecular Petri Dish)

MPD allows to perform the polymerase chain reactions (PCR) in gels.

It offers rapid diagnostics, but also brings the advantage
of absolute quantification, ensuring precise and rapid measurements without reliance on standard curves.

The immobilized cells are lysed and analysed in situ, ensuring that molecular biology is both agile
and rigorously accurate.

MPD relies on specially formulated photoactivated or thermoactivated gels, leveraging molecular diffusion
as a spatial separator.

The gel's mesh is carefully engineered to capture cells while allowing PCR reagents to diffuse,
allowing the in situ lysis and analysis of each cell.

hcPCR (High Content PCR)

Enhancing the ability to detect multiple targets within a single polymerase chain reaction, also called increasing the multiplexing level. A higher multiplexing level enables the identification of a greater number of targets of interest in a single reaction, significantly improving analysis efficiency.

This approach facilitates comprehensive diagnostics, speeds up agricultural advancements, supports environmental monitoring and plays a crucial role in the microbiota analysis.

hcPCR enhances PCR multiplexing by enabling the detection of an increased number of targets in a single experiment. Utilizing a combination of distinct probe technologies and/or fluorophores within the same optical channel, our proprietary software algorithm demultiplexes two or more overlapping signals from the PCR fluorescence data.

Unlike methods reliant on melting curves, such as High-Resolution Melting (HRM), hcPCR is able to provide quantitative measurements for each target.

FastGene

Performing polymerase chain reactions swiftly is pivotal for modern molecular biology due to the urgent demand for quick diagnostics across a wide range of applications.

Thus, increasing its speed ensures that molecular biology remains agile and responsive to both current and future challenges, making it essential for scientific progress and societal well-being.

Fastgene is the thermal module of Chronos. It features a microfluidic heat exchanger coupled with dual pressurized water tanks, enabling swift temperature transitions.

This technology allows the reaction chamber temperature switch from 60 to 95°C in under 2 seconds, significantly reducing the "thermal ramping time" to an absolute minimum.

SmartDropper

Smart Dropper is an engineered bottle cap that contains lyophilized reagents required for PCR and various other molecular biology reactions. It enables highly homogeneous mixing of the sample with the reagent in a single step, delivered in a well-controlled drop.

The Smart Dropper cap features a microporous support that holds the dried reagent, ensuring uniform mixing upon contact with the sample. Thanks to its anti-backflow system and precisely designed nozzle, both the dispensed volume and reagent resuspension yield are tightly controlled.

Protocols using Smart Dropper can achieve the performance of standard laboratory procedures with far greater simplicity. When combined with the BforCure Point-of-Care PCR chip—or used with conventional PCR containers and devices—Smart Dropper enables protocols with sensitivities reaching just a few microorganisms per milliliter at the point of need.

Ortholigo: oligonucleotide design tool

In-house software for sequence alignment and optimization of multiplex kits

Ortholigo™ is BforCure’s proprietary bioinformatics tool for in silico design of efficient and specific PCR kits. It ensures high performance in analytical and clinical validations while reducing the need for extensive experimental testing on primers and probes. Ortholigo™ generates target-specific primer and probe sequences (for viruses, bacteria, etc.) using sequence alignments and thermodynamic calculations, optimizing PCR assay.

Sequences in inclusivity and exclusivity
lists aligned for best specificity

Easily adaptable to parse metadata (ex.NCBI, SILVA,
GISAID databases) 

Implements physical models in continuous optimization to better predict reagents thermodynamics

Future developments will include connectivity with various systems for automation of processes in the lab

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